Antitumor activity of miR-188-3p in gastric cancer is achieved by targeting CBL expression and inactivating the AKT/mTOR signaling

Figure 6 Effects of miR-188-3p expression on the regulation of gastric cancer cell proliferation viaCBL downregulation. A: Western blot. BGC-823 cells were transfected with m-NC and vector, miR-188-3p/CBL cDNA, miR-188-3p mimics + vector-only and were then subjected to Western blotting. The expression of becline1 and phosphorylation of the Akt and mTOR proteins was decreased in gastric cancer cells after knockdown of CBL using CBL siRNA; B: Western blot. CBL, BGC-823 and AGS cells were seeded in 10 cm cell culture dishes for transfection with miR-188-3p mimic, inhibitor, or control (NC) plasmid, after which Western blot was performed; C: Nude mouse model of gastric cancer cell xenograft assay. The images of nude mouse tumor cell xenografts after injection with BGC-823/m-NC, BGC-823/miR-188-3p/CBL, or BGC-823/miR-188-3p cells; D: Tumor cell xenograft size. After tumor cell inoculation, tumor size was measured every 7 d in each group, m-NC vs miR-188-3p/CBL, ^bP < 0.001; miR-188-3p vs miR-188-3p/CBL cDNA, ^cP < 0.01.