Antitumor activity of miR-188-3p in gastric cancer is achieved by targeting CBL expression and inactivating the AKT/mTOR signaling

Figure 5 CBL antagonization of miR-188-3p reduced gastric cancer cell proliferation and induced gastric cancer cell apoptosis and autophagy in vitro. A and B: Flow cytometry. AGS and BGC-823 cells were transfected with miR-188-3p mimics, miR-188-3p/CBL cDNA, or m-NC, miR-188-3p mimics + vector-only, after which the flow cytometric apoptosis assay was performed. ^cP < 0.001; C: qRT-PCR. AGS and BGC-823 cells were transfected with miR-188-3p mimics, miR-188-3p/CBL cDNA, or m-NC, miR-188-3p mimics + vector-only, after which qRT-PCR analysis was conducted. ^cP < 0.001; D: CCK-8 assay. AGS and BGC-823 cells were seeded for transfection with miR-188-3p mimics, miR-188-3p/CBL cDNA, or m-NC, miR-188-3p mimics + vector-only, after which the CCK-8 assay was performed.