Potential of damage associated molecular patterns in synergising radiation and the immune response in oesophageal cancer

Figure 3 HMGB1 and calreticulin are expressed at a higher level under 5% oxygenation with no glucose in the OE33P cell line, with a higher expression of HMGB1 under normal oxygenation in the OE33R cell line. OE33P and OE33R cell lines were screened for the surface expression of HMGB1 and CRT following culture in glucose depleted media by flow cytometry. 21% = normal oxygenation, 5% = mild hypoxia, 0.5% = severe hypoxia. Bolus dosing was administered once daily over three consecutive days. Staining of cancer cells took place 24hrs after last fraction of radiation. Tukey’s multiple comparison testing. Graph shows mean % expression (± SEM) (n = 3). ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001 vs oxygen levels for each radiation dosing regimen; ^eP < 0.05, ^fP < 0.01, ^gP < 0.001, ^hP < 0.0001 vs dosing with 0 Gy at 21% O₂; ⁱP < 0.05, ^jP < 0.01, ^kP < 0.001 vs dosing with 0 Gy at 5% O₂; ^lP < 0.05, ^mP < 0.01, ⁿP < 0.001 vs dosing with 0 Gy at 0.5% O₂.