ENTPD1-AS1–miR-144-3p-mediated high expression of COL5A2 correlates with poor prognosis and macrophage infiltration in gastric cancer

Figure 5 Long noncoding RNA ENTPD1-AS1 regulated the expression of COL5A2 through sponging miR-144-3p. A: Construction of dual luciferase reporter plasmids, including wild type (wt) and mutant (mut) of 3′UTR COL5A2 and ENTPD1-AS1; B: dual luciferase reporter assay to evaluate the direct interaction of COL5A2 and miR-144-3p in 293T; C: Dual luciferase reporter assay to evaluate the direct interaction of COL5A2 and miR-144-3p in human gastric adenocarcinoma cell line (AGS); D: dual luciferase reporter assay to detect the interaction of ENTPD1-AS1 and miR-144-3p in 293T; E: dual luciferase reporter assay to detect the interaction of ENTPD1-AS1 and miR-144-3p in AGS; F: The Transfection efficiency of miR-144-3p in AGS was evaluated; G: Effect of miR-144-3p on the expression of COL5A2, detected by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR); H: The Transfection efficiency of ENTPD1-AS1 in AGS was evaluated; I: Effect of ENTPD1-AS1 on the expression of miR-144-3p in AGS, detected by qRT-PCR. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. AGS: Human gastric adenocarcinoma cell line; NC: Negative controls.