Transcription factor glucocorticoid modulatory element-binding protein 1 promotes hepatocellular carcinoma progression by activating Yes-associate protein 1

Figure 5 Effects of the interaction between glucocorticoid modulatory element-binding protein 1 and Yes-associate protein 1 on the biological functions of hepatocellular carcinoma CDX mice models. A and B: HepG2 CDX mice model. Sh-NC, sh-GMEB1, oe-NC, oe-YAP1, sh-GMEB1+oe-YAP1 cells were injected subcutaneously into the right axilla of mice (n = 5/group) and tumor size was measured every 3 d. Tumor volume was calculated using the formula: V = (L x W²)/2. 30 days post injection, mice were sacrificed and tumors were peeled and weighted. C and D: HepG2 metastasis mice model. Sh-NC, sh-GMEB1, oe-NC, oe-YAP1, sh-GMEB1+oe-YAP1 cells were injected into nude mice (n = 5/group) via tail vein, respectively. Mice were sacrificed 60 d after injection, and number of metastatic foci on the lungs of each group were photographed and counted. ^aP < 0.05, ^bP < 0.01, and ^eP < 0.001 vs sh-NC group; ^dP < 0.01 and ^fP < 0.001 vs oe-NC group. NS: Not significant. GMEB1: Glucocorticoid modulatory element-binding protein 1; YAP1: Yes-associate protein 1.