Transcription factor glucocorticoid modulatory element-binding protein 1 promotes hepatocellular carcinoma progression by activating Yes-associate protein 1

Figure 4 Effects of the interaction between glucocorticoid modulatory element-binding protein 1 and Yes-associate protein 1 on the biological functions of hepatocellular carcinoma cells. A and B: qRT-PCR and Western blot were utilized to detect YAP1 mRNA and protein expression in HepG2 cells transfected with NC, pcDNA-GMEB1, pcDNA-GMEB1+si-YAP1 and Huh7 cells transfected with si-NC, si-GMEB1#1, si-GMEB1#1+pcDNA-YAP1. C: Evaluation of HepG2 and Huh7 cell proliferation after transfection through the CCK-8 method. D and E: Transwell assays were conducted to detect HepG2 and Huh7 cell migration and invasion after transfection. F: Detection of HepG2 and Huh7 cell apoptosis after transfection via flow cytometry. ^aP < 0.05; ^bP < 0.01; and ^eP < 0.001. GMEB1: Glucocorticoid modulatory element-binding protein 1; YAP1: Yes-associate protein 1.