Transcription factor glucocorticoid modulatory element-binding protein 1 promotes hepatocellular carcinoma progression by activating Yes-associate protein 1

Figure 3 Glucocorticoid modulatory element-binding protein 1 targets and binds to Yes-associate protein 1. A: The JASPR database (http://jaspar.genereg.net/) was employed to predict the binding site of glucocorticoid modulatory element-binding protein 1 (GMEB1) and Yes-associate protein 1 (YAP1) promoter region. B: The StarBase database was employed to analyze the correlation between GMEB1 and YAP1 expression in hepatocellular carcinoma (HCC) tissues. C: Verification by dual-luciferase reporter gene assay of the binding relationship between GMEB1 and YAP1 promoter sequence. D: Detection via ChIP-qPCR assay of the binding of GMEB1 to the YAP1 promoter region. E and F: qRT-PCR and Western blotting were conducted to detect the effects of GMEB1 overexpression or knockdown on YAP1 mRNA and protein expression in HepG2 and Huh7 cells. G: Detection of YAP1 mRNA expression in HCC and para-tumorous tissues by qRT-PCR. H: Pearson correlation analysis of the correlation between GMEB1 mRNA and YAP1 mRNA expression in 55 cases of HCC tissues. ^bP < 0.01; and ^eP < 0.001. GMEB1: Glucocorticoid modulatory element-binding protein 1; YAP1: Yes-associate protein 1.