5’tiRNA-Pro-TGG, a novel tRNA halve, promotes oncogenesis in sessile serrated lesions and serrated pathway of colorectal cancer

Figure 4 Transfer RNA halves-1:33-Pro-TGG-1 is associated with lesion size and promotes oncogenesis in colorectal cancer cells. A: Correlation analysis of the lesion sizes and expressions of transfer RNA halves (tiRNA)-1:33-Pro-TGG-1 of SSLs. The X-axis represents the diameter of the lesion and the Y-axis represents the expression level of tiRNA-1:33-Pro-TGG-1. B: Lesions in the tiRNA-1:33-Pro-TGG-1 high-expression group are larger than that of the low-expression group. Lesions are divided into high- and low-expression groups based on the median tiRNA-1:33-Pro-TGG-1 expression levels. The lesion sizes are compared between the two groups. C and D: Correlation analysis of lesion sizes and the expressions of tiRNA-1:34-Thr-TGT-4-M2 and tiRNA-1:33-Gly-CCC-2 of SSLs. E: 5′tiRNA-Pro-TGG is a type of 5′tiRNA that originated in tRNA-Pro-TGG-1. F: 5′tiRNA-Pro-TGG knockdown suppressed the proliferation of RKO cells compared to that of the scramble control as determined by CCK-8 assays. G and H: Wound-healing assays demonstrated that the overexpression of 5′tiRNA-Pro-TGG promotes cell migration and knockdown of 5′tiRNA-Pro-TGG inhibits cell migration when compared to those of the corresponding scramble control, respectively. The asterisk indicates a significant difference between the two groups (^aP < 0.05). SSLs: Sessile serrated lesions; tiRNA: Transfer RNA halves.