BZD9L1 benzimidazole analogue hampers colorectal tumor progression by impeding angiogenesis

Figure 7 Quantitative polymerase chain reaction analyses of murine SIRT1, mSIRT2 and endothelial cell markers in HCT116 tumor xenograft in nude mice. A: BZD9L1 did not significantly affect murine SIRT1 (mSIRT1) gene expression in the colorectal cancer xenograft tumors but down-regulated mSIRT2 at low (50 mg/kg) and high (250 mg/kg) doses and reduced human SIRT1 (hSIRT1) and hSIRT2 gene expression at 250 mg/kg, compared to vehicle control; B: BZD9L1 at the high dose significantly reduced cluster of differentiation 31 (CD31) gene expression. Both low and high doses of BZD9L1 decreased mCD34 gene expression compared to vehicle control. Statistical analysis ^aP < 0.05; ^bP < 0.01; ^cP < 0.001), one-way ANOVA with Bonferroni posthoc test, n = 6 animals per group) using GraphPad Prism 9.4.0. Error bars represent SEM. VC: Vehicle control.