BZD9L1 benzimidazole analogue hampers colorectal tumor progression by impeding angiogenesis

Figure 5 Refreshed conditioned media from Ea. Hy 926 endothelial cells 48 h post-treatment with Sunitinib positive control, BZD9L1 at 2.5 μM, 5.0 μM, or negative control for 48 h prior, were subjected to Quantibody Human Angiogenesis array and indirect co-culture with HCT116 colorectal cancer spheroids. A: BZD9L1 at 5 μM down-regulated angiogenin, fibroblast growth factor, platelet-derived growth factor and placental growth factor cytokine concentrations in Ea.Hy 926 conditioned media; B: The endothelial conditioned media from BZD9L1 or sunitinib positive control-treated groups impeded HCT116 tumor invasion at 72 h. Statistical analysis (^aP < 0.05; ^bP < 0.01; ^cP < 0.001), one-way ANOVA with Bonferroni posthoc test, n = 2 independent experiments for protein array analysis and n = 3 independent replicates for co-culture analysis using GraphPad Prism 9.4.0. Error bars represent SEM. NC: Negative control. bFGF: Fibroblast growth factor; PDGF-BB: Platelet-derived growth factor; PIGF: Placental growth factor; NC: Negative control.