Circ_0003356 suppresses gastric cancer growth through targeting the miR-668-3p/SOCS3 axis

Figure 3 Circ_0003356 directly binds to miR-668-3p. A: Fluorescence in situ hybridization assays were used to detect the sub-cellular localization of circ_0003356 in AGS and HGC-27 cells; B: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of circ_0003356 in the nucleus and cytoplasm; C: Binding sequences between circ_0003356 and miR-668-3p predicted by circinteractome; D: Interaction between circ_0003356 and miR-668-3p in AGS and HGC-27 cells validated by RIP-qRT-PCR; E: interaction between circ_0003356 and miR-668-3p in AGS and HGC-27 cells validated by RNA pull-down assays; F: Interaction between circ_0003356 and miR-668-3p in AGS and HGC-27 cells validated by dual-luciferase reporter assays; G: Relative expression of miR-668-3p detected by qRT-PCR in tumor tissues and normal tissues; H: Correlation between circ_0003356 and miR-668-3p in gastric cancer tissues analyzed by Pearson’s correlation analysis. ^cP < 0.001.