Circ_0003356 suppresses gastric cancer growth through targeting the miR-668-3p/SOCS3 axis

Figure 2 Upregulation of circ_0003356 promotes cell apoptosis and suppresses proliferation, migration, invasion, and epithelial-mesenchymal transition of AGS and HGC-27 cells. A: Relative expression of circ_0003356 in AGS and HGC-27 cells detected by quantitative real-time polymerase chain reaction; B: Cell viability in AGS and HGC-27 cells assessed by CCK-8 assay; C: Proliferation of AGS and HGC-27 cells assessed by EdU assays. D: Apoptosis of AGS and HGC-27 cells assessed by flow cytometry; E: Migration of AGS and HGC-27 cells detected by wound healing assays; F: Invasion of AGS and HGC-27 cells detected by transwell assay; G: Levels of epithelial-mesenchymal transition-related proteins (E-cadherin and N-cadherin) in AGS and HGC-27 cells determined using western blotting. ^bP < 0.01, ^cP < 0.001.