Xiaojianzhong decoction prevents gastric precancerous lesions in rats by inhibiting autophagy and glycolysis in gastric mucosal cells

Figure 7 Xiaojianzhong decoction inhibited Unc-51 Like kinase 1 and p53/AMP-activated protein kinase pathway in N-methyl-N’-nitro-N-nitrosoguanidine-induced gastric precancerous lesions rats. A and B: Unc-51 like kinase 1 (ULK1) and p53 protein expression was determined using immunofluorescence (n = 5); ULK1, p53 proteins positive score was determined using Image J; C: ULK1 mRNA expression was determined using real-time polymerase chain reaction analysis (n = 3); D-G: Western blot analysis was performed to detect p-p53, p-AMP-activated protein kinase, p-ULK1 (Ser555), and p-ULK1 (Ser317) protein expression (n = 3); H and I: ULK1 and p53 protein expression was determined using immunofluorescence (n = 5). Data are expressed as mean ± SD. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs control group; ^dP < 0.05, ^eP < 0.01, ^fP < 0.001 vs model group. XJZ-L: Low dose of Xiaojianzhong decoction; XJZ-M: Middle dose of Xiaojianzhong decoction; XJZ-H: High dose of Xiaojianzhong decoction; ULK1: Unc-51 like kinase 1.