Xiaojianzhong decoction prevents gastric precancerous lesions in rats by inhibiting autophagy and glycolysis in gastric mucosal cells

Figure 6 Effects of Xiaojianzhong decoction on hypoxia-induced glycolysis in gastric mucosal epithelial cells. A-C: Hypoxia-induced glycolysis-related protein hypoxia-inducible factor 1α (HIF-1α), CD147 expression was determined using immunofluorescence (n = 5); HIF-1α, CD147 proteins positive score was determined using Image J; D-F: CD147, monocarboxylate transporter (MCT1), and MCT4 mRNA expression was determined using real-time polymerase chain reaction analysis (n = 3); G and H: Sirtuin 6 (SIRT6) expression was determined using immunohistochemistry (n = 5); SIRT-6 positive score was determined using Image J. Data are expressed as mean ± SD. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs control group; ^dP < 0.05, ^eP < 0.01, ^fP < 0.001 vs model group. XJZ-L: Low dose of Xiaojianzhong decoction; XJZ-M: Middle dose of Xiaojianzhong decoction; XJZ-H: High dose of Xiaojianzhong decoction; HIF-1α: Hypoxia-inducible factor 1α; MCT: Monocarborxylat transporter.