Xiaojianzhong decoction prevents gastric precancerous lesions in rats by inhibiting autophagy and glycolysis in gastric mucosal cells

Figure 4 Xiaojianzhong decoction up-regulates phosphatidylinositol 3-kimase/protein kinase B/mammalian target of rapamycin signaling pathway in N-methyl-N’-nitro-N-nitrosoguanidine-induced gastric precancerous lesions rats. A-C: Western blot analysis was performed to detect phosphorylated-phosphatidylinositol 3-kimase (p-PI3K), phosphorylated-protein kinase B (p-AKT), and phosphorylated-mammalian target of rapamycin (p-mTOR) protein expression (n = 3); D-F: PI3K, AKT, and mTOR mRNA expression was determined using real-time polymerase chain reaction analysis (n = 3). Data are expressed as mean ± standard deviation. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs control group; ^dP < 0.05, ^eP < 0.01, ^fP < 0.001 vs model group. XJZ-L: Low dose of Xiaojianzhong decoction; XJZ-M: Middle dose of Xiaojianzhong decoction; XJZ-H: High dose of Xiaojianzhong decoction.