Xiaojianzhong decoction prevents gastric precancerous lesions in rats by inhibiting autophagy and glycolysis in gastric mucosal cells

doi:10.4251/wjgo.v15.i3.464

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World Journal of Gastrointestinal Oncology

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1948-5204

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Basic Study

World J Gastrointest Oncol. Mar 15, 2023; 15(3): 464-489
Published online Mar 15, 2023. doi: 10.4251/wjgo.v15.i3.464

Table 1 Primer pair sequences

Gene	Primer	Sequence, 5’-3’	PCR products
B-actin	Forward	CACGATGGAGGGGCCGGACTCATC	240 bp
B-actin	Reverse	TAAAGACCTCTATGCCAACACAGT	240 bp
Rat PI3K	Forward	GCAACAAGTCCTCTGCCAAA	222 bp
Rat PI3K	Reverse	ACGTAATAGAGGAGCTGGGC	222 bp
Rat AKT	Forward	GCTCTTCTTCCACCTGTCTCG	186 bp
Rat AKT	Reverse	CACAGCCCGAAGTCCGTTA	186 bp
Rat mTOR	Forward	GAGATACGCCGTCATTCCT	199 bp
Rat mTOR	Reverse	ATGCTCAAACACCTCCACC	199 bp
Rat HIF-1α	Forward	GCGGCGAGAACGAGAAGAAAAATAG	129 bp
Rat HIF-1α	Reverse	GAAGTGGCAACTGATGAGCAAG	129 bp
Rat MCT1	Forward	TGTATGCCGGAGGTCCTATC	177 bp
Rat MCT1	Reverse	AGTTGAAAGCAAGCCCAAGA	177 bp
Rat MCT4	Forward	ACGGCAGGTTTCATAACAGG	162 bp
Rat MCT4	Reverse	GCCATACGAGATCCCAAAGA	162 bp
Rat Bnip-3	Forward	GGTCAAGTCGGCCAGAAAAT	207 bp
Rat Bnip-3	Reverse	TTGTCAGACGCCTTCCAATG	207 bp
Rat LC3	Forward	AAAATGGGGCACGGATGAAG	155 bp
Rat LC3	Reverse	GCAGGTCTTCAAAATGCCCA	155 bp
Rat CDX-2	Forward	TCTCCGAGAGGCAGGTTAAA	186 bp
Rat CDX-2	Reverse	GCAAGGAGGTCACAGGACTC	186 bp
Homo ULK1	Forward	TTATCATGTCCCAGCACTACGAT	242 bp
Homo ULK1	Reverse	AAATTCATCAAAGTCCATGCGGT	242 bp
Rat CD147	Forward	GGCACCATCGTAACCTCTGT	211 bp
Rat CD147	Reverse	CAGGCTCAGGAAGGAAGATG	211 bp
Rat Beclin-1	Forward	GAGGTACCGACTTGTTCCCT	225 bp
Rat Beclin-1	Reverse	CCTTTCTCCACGTCCATCCT	225 bp

AKT: Protein kinase B; Beclin-1: Moesin-like BCL2-interacting protein 1; Bnip-3: B cell lymphoma/Leukemia-2 and adenovirus E1B19000 interacting protein 3; HIF-1α: Hypoxia-inducible factor-1α; LC-3: Microtubule associated protein 1 light chain 3; PCR: Polymerase chain reaction; PI3K: Phosphatidylinositol 3-kimase; MCT: Monocarboxylate transporter; mTOR: Mammalian target of rapamycin signaling pathway; ULK1: Unc-51 like kinase 1.

Citation: Zhang JX, Bao SC, Chen J, Chen T, Wei HL, Zhou XY, Li JT, Yan SG. Xiaojianzhong decoction prevents gastric precancerous lesions in rats by inhibiting autophagy and glycolysis in gastric mucosal cells. World J Gastrointest Oncol 2023; 15(3): 464-489
URL: https://www.wjgnet.com/1948-5204/full/v15/i3/464.htm
DOI: https://dx.doi.org/10.4251/wjgo.v15.i3.464