Potent bromodomain and extraterminal domain inhibitor JAB-8263 suppresses MYC expression and exerts anti-tumor activity in colorectal cancer models

Figure 3 JAB-8263 dose-dependently induced cell cycle arrest and apoptosis in the MC38 cell line. After incubation with different concentrations of JAB-8263 (0, 0.032, 0.16, 0.8, 4, 20 and 100 nmol/L) for 3 d and 5 d, MC38 cells were collected and analyzed for cell cycle and apoptosis assays by flow cytometry. A and B: JAB-8263 dose-dependently decreased the G2/M phase ratio and increased the G0 prophase ratio in MC38 cells, indicating that JAB-8263 induced cells to arrest in the G0 phase; C and D: JAB-8263 dose-dependently induced apoptosis in MC38 after treatment for 3 d and 5 d. All experiments were performed in triplicate. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs DMSO control. PI: Propidium iodide.