Potent bromodomain and extraterminal domain inhibitor JAB-8263 suppresses MYC expression and exerts anti-tumor activity in colorectal cancer models

Figure 1 JAB-8263 dose-dependently suppressed colorectal cancer cell proliferation and colony formation in vitro. A: Human colorectal cancer cell lines including HT29, DLD1, Colo205, H716, SW837 and H508 were treated with JAB-8263 for 6 d, and the proliferation was dose-dependently suppressed; B: The MC38 mouse cell line was treated with JAB-8263 for 6 d, and the proliferation was dose-dependently suppressed; C: The IC₅₀ values of HT-29, DLD-1, Colo205, H716, SW837, H508 and MC38 were 0.15, 1.24, 0.19, 0.09, 0.57, 0.14 and 1.25 μmol/L. Colony formation assays for six colorectal cancer cell lines including MC38, HT29, H508, SW837 and DLD1 were treated with various concentrations of JAB-8263 for 5 d. Cell proliferation in all cell lines was dose-dependently suppressed. All experiments were performed in triplicate. Conc.: Concentration.