microRNA-627-5p inhibits colorectal cancer cell proliferation, migration and invasion by targeting Wnt2

Figure 3 The direct binding relationship between microRNA-627-5p and Wnt2. A: Schematic illustration of the predicted binding sites between microRNA-627-5p (miR-627-5p) and Wnt2 mRNA; B: Dual luciferase reporter assays in HEK-293T cells. Experimental group: NC mimics + pcDNA-Wnt2-WT, miR-627-5p mimics + pcDNA-Wnt2-WT, NC mimics + pcDNA-Wnt2-MUT, miR-627-5p mimics + pcDNA-Wnt2-MUT; C: The transfection efficiency of miR-627-5p mimics in SW480 and HCT116 cells; D: The effects of miR-627-5p overexpression on the transcript expression levels of Wnt2 in SW480 and HCT116 cells; E: The effects of miR-627-5p overexpression on the protein expression levels of Wnt2 in SW480 and HCT116 cells. 3’ UTR: 3’-untranslated region; NS: Not significant; ^bP < 0.01; ^cP < 0.001.