Increased CD4/CD8 Lymphocyte ratio predicts favourable neoadjuvant treatment response in gastric cancer: A prospective pilot study

Figure 3 Association between biomarker expression and neoadjuvant 5-fluorouracil, leucovorin, oxaliplatin and docetaxel chemotherapy. A: Paired boxplots for biomarker expression pre- and post-neoadjuvant chemotherapy. Each coloured point and line correspond to a single patient. Boxplots in grey represent the distribution of expression for all patients before and after chemotherapy. Paired Wilcoxon P value is present in each plot; B: Boxplot comparison of expression for each respective biomarker between treatment response (purple) and incomplete response (green). The immunohistochemistry (IHC) biomarker is labeled on the heading of each graph. The y-axis represents IHC score, which is the percent of positive stained cells for Galectin-3, CD4, CD8 and E-cadherin and the H-score for ECAD H-score plot; C: Forest plot for metrics of ElasticNet models. Models were constructed using the treatment response as the dependent variable and the corresponding independent variable(s) identified in the variable column. The plot represents the out-of-sample accuracy (blue square) and 95% confidence intervals (whiskers) for models estimated from 1000 bootstraps with replacement. The no information rate, defined as the maximum accuracy of a classifier if it predicted the majority class every time, is shown by the solid and dotted vertical lines for univariable and multivariable models, respectively; D: Barplot of model coefficients from multivariable glmnet model. The y-axis represents model covariates and the x-axis the coefficient value. Treatment response is related to increasing covariate values or decreasing covariate values for positive and negative coefficients, respectively. The absolute importance of the coefficient is shown in blue according to the scale legend; E: Lineplot illustrating monte-carlo simulations for two-sample Wilcoxon sample size calculations. The y-axis is the empirical power and the x-axis is the sample size in each group. Each coloured line corresponds to a biomarker labelled according to the legend.