Cancerous inhibitor of protein phosphatase 2A enhances chemoresistance of gastric cancer cells to oxaliplatin

Figure 8 Chemical inhibition of protein kinase B signaling significantly increased sensitivity to oxaliplatin in human gastric cancer cells. MKN-45 and AGS cells were added to inhibitor of protein kinase B (MK-2206; 20 μM) for 2 h. A: Immunoblotting was performed to evaluate the corresponding protein expression; B: The phosphorylated protein kinase B (P-Akt) protein levels were quantified via densitometry; C and D: The pretreated cells were exposed to oxaliplatin at different concentrations for 24 h, and the viability was examined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium assay. The results consisted of three independent tests. The MK-2206-treated group vs the control group. ^aP < 0.05. ^bP < 0.01. Akt: Protein kinase B.