Cancerous inhibitor of protein phosphatase 2A enhances chemoresistance of gastric cancer cells to oxaliplatin

Figure 4 Small interference RNA targeting cancerous inhibitor of protein phosphatase 2A effectively downregulated expression and decreased cell proliferation in human gastric cancer cells. MKN-45 and AGS cells were transfected with negative control small interference RNA (siRNA) or cancerous inhibitor of protein phosphatase 2A (CIP2A) siRNA for 48 h. A: CIP2A expression in MKN-45 cells was determined via real-time quantitative reverse transcription (RT) PCR; B: CIP2A expression in AGS cells was determined via real-time quantitative RT PCR; C and D: CIP2A expression was determined via immunoblotting; E and F: Data were quantified using a spectrophotometer. The CIP2A siRNA group vs the control siRNA group. G and H: The downregulation of CIP2A expression could decrease cell proliferation in MKN-45 and AGS cells based on the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium assay. Data showed a significant reduction in the proliferation of CIP2A siRNA-treated cells. The results were three independent tests. ^bP < 0.01.