Hsa_circ_0136666 mediates the antitumor effect of curcumin in colorectal carcinoma by regulating CXCL1 via miR-1301-3p

Figure 7 Downregulating CXCL1 abrogated the influence of miR-1301-3p knockdown on curcumin-triggered colorectal carcinoma cell proliferation and apoptosis. A: Real-time quantitative PCR assay measurement of miR-1301-3p in inhibitor NC and miR-1301-3p inhibitor-transfected SW480 and SW620; B: Western blot assay of CXCL1 protein in si-NC or si-CXCL1-transfected SW480 and SW620; C: Western blot assay of CXCL1 protein in SW480 and SW620 after inhibitor NC, miR-1301-3p inhibitor, miR-1301-3p inhibitor + si-NC and miR-1301-3p inhibitor + si-CXCL1 transfection; D-L: Colorectal carcinoma cells were intervened by curcumin after transfection; D: Cell counting kit-8 assay of cell viability; E and F: Colony-forming cell assay of colony number; G: 5-Ethynyl-2’-deoxyuridine assay of positive cells; H: Flow cytometry assay of cell cycle distribution; I and J: Flow cytometry assay of apoptosis; K and L: Western blot assay of Bcl-2 and Bax protein levels. ¹P < 0.05.