Hsa_circ_0136666 mediates the antitumor effect of curcumin in colorectal carcinoma by regulating CXCL1 via miR-1301-3p

Figure 6 CXCL1 was miR-1301-3p’s direct target. A: Putative binding sequences between miR-1301-3p and CXCL1 3’UTR, and mutant sites in CXCL1 3’UTR mut; B: Validation of prediction by dual-luciferase reporter assay; C: Western blot assay quantification of CXCL1 protein in SW480, SW480 + DMSO, SW480 + curcumin, SW620, SW620 + DMSO, and SW620 + curcumin; D: Western blot assay quantification of CXCL1 protein in SW480 and SW620 transfected with vector, oe-hsa_circ_0136666, oe-hsa_circ_0136666 + mimic NC, and oe-hsa_circ_0136666 + miR-1301-3p mimic. ¹P < 0.05. 3’UTR: 3’ untranslated region; wt: Wild-type; mut: Mutant-type.