Hsa_circ_0136666 mediates the antitumor effect of curcumin in colorectal carcinoma by regulating CXCL1 via miR-1301-3p

Figure 5 miR-1301-3p overturned the effect of hsa_circ_0136666 on growth and apoptosis in curcumin-treated colorectal carcinoma cells. A: Real-time quantitative PCR assays quantified miR-1301-3p levels in SW480 and SW620 transfected with vector, oe-hsa_circ_0136666, oe-hsa_circ_0136666+mimic NC, and oe-hsa_circ_0136666 + miR-1301-3p mimic; B-J: Colorectal carcinoma cells were intervened by curcumin after transfection; B: Cell counting kit-8 assays measured cell viability; C and D: Colony-forming cell assays determined the colony number; E: 5-Ethynyl-2’-deoxyuridine assays examined positive cell count; F: Flow cytometry assays determined cell cycle distribution; G and H: Flow cytometry assays tested apoptosis; I and J: Western blot assays quantified Bcl-2 and Bax protein levels. ¹P < 0.05.