Hsa_circ_0136666 mediates the antitumor effect of curcumin in colorectal carcinoma by regulating CXCL1 via miR-1301-3p

Figure 4 Hsa_circ_0136666 directly bound to miR-1301-3p. A: Real-time quantitative PCR (RT-qPCR) measured relative levels of 3 miRNA candidates in SW480 and SW620 lysates; B: RNA immunoprecipitation assays assessed miR-1301-3p endogenously associated with hsa_circ_0136666 in SW480 and SW620 extracts; C: Binding loci between hsa_circ_0136666 and miR-1301-3p and the hsa_circ_0136666 mut sequence; D: The binding relationship was verified by a dual-luciferase reporter assay; E: RT-qPCR assays quantified miR-1301-3p in SW480, SW480 + DMSO, SW480 + curcumin, SW620, SW620 + DMSO, and SW620 + curcumin. ¹P < 0.05. wt: Wild-type; mut: Mutant-type.