Dopamine and cyclic adenosine monophosphate-regulated phosphoprotein with an apparent Mr of 32000 promotes colorectal cancer growth

Figure 6 Effect of dopamine and cyclic adenosine monophosphate regulated phosphoprotein with an apparent Mr of 32000 on progression is partially dependent on the phosphoinositide 3-kinase/AKT signaling pathway. A: Our group examined the differences in mRNA expression between the normal control and dopamine and cyclic adenosine monophosphate regulated phosphoprotein with an apparent Mr of 32000 (DARPP-32) groups using RNA sequencing (RNA-seq) analysis; B: Heat map of a DARPP-32 downstream target assessed by RNA-seq analysis. Red indicates upregulation of the corresponding gene and green indicates downregulation; C: Bubbly graph of Gene Ontology enrichment terms; D: Analysis of the DARPP-32 level Kyoto Encyclopedia of Genes and Genomes data; E: Western blot analysis of DARPP-32, AKT, p-AKT, phosphoinositide 3-kinase (PI3K) and phosphorylated PI3K proteins in DARPP-32-overexpressing cells or DARPP-32 small interfering RNA (siRNA)-infected cells. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as a loading control. NC: Negative control.