Hsa_circ_0001658 accelerates the progression of colorectal cancer through miR-590-5p/METTL3 regulatory axis

Figure 3 Circ_0001658 targets miR-590-5p. A: Subcellular localization of circ_0001658 in HT29 and SW480 cells was under the detection of a nuclear mass separation assay; B: Online database Circular RNA Interactome predicted the binding site of circ_0001658 to miR-590-5p; C-D: Circ-wild-type and circ-mutant type were co-transfected with miR-negative control and miR-590-5p, respectively, into HT29 and SW480 cells, and the activity was probed by a dual luciferase reporter gene assay; E: The effect of circ_0001658 overexpression or knockdown on miR-590-5p expression was probed by quantitative real-time polymerase chain reaction (qRT-PCR); F: The expression of miR-590-5p in normal tissues and colorectal cancer (CRC) tissues was under the assay of qRT-PCR. G: Pearson’s correlation analysis detected the correlations between circ_0001658 and miR-590-5p expression in CRC tissues. ^bP < 0.01; ^cP < 0.001. ns: No significance; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; WT: Wild-type; MUT: Mutant type; miR-NC: miRNA negative control; circ-OE: Circ_0001658 plasmid; si-NC: siRNA negative control.