Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

Figure 3 Evaluations of CD8⁺ T cell function. Activated CD8⁺ T cells were co-cultured with Huh-7 cells for 3 d. Flow cytometry was used to detect the expression of indicated molecules in CD8⁺ T cells. A: PD-1; B: PRF1; C: Granzyme B (GZMB). D and E: Quantitative real-time polymerase chain reaction was used to detect the mRNA levels in CD8⁺ T cells (D: PRF1; E: GZMB). The data represent the mean ± SE; ^aP < 0.05; ^bP < 0.01; ^cP < 0.001; ^dP < 0.0001.