N-myc downstream regulated gene 1 inhibition of tumor progression in Caco2 cells

Figure 4 The results of quantitative polymerase chain reaction and western blotting after N-myc downstream regulated gene 1 over-expression and knockout. A: The mRNA levels of N-myc downstream regulated gene 1 (NDRG1) were detected using real-time polymerase chain reaction. The relative levels of NDRG1 mRNA were calculated using the ΔΔCt method, and normalized to GAPDH expression; B-D: Representative western blot bands and statistical results of NDRG1 protein expression normalized to β-tubulin. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs control cells. Experiments were repeated three times.