E3 ubiquitin ligase TRIM55 promotes metastasis of gastric cancer cells by mediating epithelial-mesenchymal transition

Figure 2 TRIM55 expression in gastric cancer cells. A: The expression of TRIM protein was detected in gastric cancer (GC) cells by western blot. The histogram shows the expression of TRIM55 in GC cells was stronger than that in gastric mucosa cell line; B: The interference efficiency of TRIM55 small interfering RNA (siRNA) in SGC7901 cells was detected by Western blot; C: Quantitative real-time-polymerase chain reaction assay was performed to determine TRIM55 expression after transfection with siRNA; D: The up-regulation of TRIM55 expression in HGC27 cells was detected by Western blot; E: TRIM55 mRNA expression level in HGC27 after transfection with the TRIM55 plasmid. ^bP < 0.01; ^cP < 0.001.