MiR-30e-3p inhibits gastric cancer development by negatively regulating THO complex 2 and PI3K/AKT/mTOR signaling

Figure 5 Knockdown of THO complex 2 suppresses malignant behaviors of gastric cancer cells. AGS or BGC-823 cells were transfected with siNC or siTHO complex 2 (siTHOC2)-1/2 to knockdown the expression of THOC2. A: The protein expression of THOC2 was analyzed by western blotting 48 hours later; B and C: Colony formation assay was conducted to evaluate the cell proliferation; D: CCK-8 assay was conducted to evaluate cell viability; E-H: Transwell assay was conducted using transwell chamber with or without Matrigel to evaluate the cell migration (E and F) and invasion (G and H); I: Expression of E-cadherin, N-cadherin, and vimentin in AGS or BGC-823 was determined using western blotting. n = 3 for each group. ^bP < 0.01.