MiR-30e-3p inhibits gastric cancer development by negatively regulating THO complex 2 and PI3K/AKT/mTOR signaling

Figure 4 THO complex 2 is a direct target of miR-30e-3p in gastric cancer cells. A: Bioinformatics analysis predicted the potential targets of miR-30e-3p in gastric cancer (GC) cells; B: The mRNA expression level of candidate genes in GC after transfecting with miR-30e-3p inhibitor; C and D: The protein expression level of candidate genes in GC after transfecting with miR-30e-3p inhibitor; E: The expression of THO complex 2 (THOC2) in GSE-1 cells after transfecting with miR-30e-3p; F: The putative binding sequences between miR-30e-3p and 3’-UTR of THOC2 was shown. AGS or BGC-823 cells were co-transfected with luciferase reporter vector, with or without miR-30e-3p mimics. The relative luciferase activity in AGS or BGC-823 cells was analyzed 48 hours later. n = 3 for each group. G: The expression of THOC2 in human GC cell lines and control GES-1 cells were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. n = 3 for each group; H: The expression of THOC2 in GC tissues and adjacent non-tumor normal tissues were analyzed by qRT-PCR. n = 20 for each group; I: Western blot was performed to examine THOC2 protein expression in human GC tissues and adjacent non-tumor normal tissues. n = 20 for each group. J: Pearson correlation analysis was performed to evaluate the relationship between THOC2 mRNA and miR-30e-3p expression in GC tissues. n = 20. ^bP < 0.01.