MiR-30e-3p inhibits gastric cancer development by negatively regulating THO complex 2 and PI3K/AKT/mTOR signaling

Figure 3 Inhibition of miR-30e-3p promotes the malignant behaviors of gastric cancer cells. AGS or BGC-823 cells were transfected with negative control, miR-30e-3p inhibitors, or left untreated (control). A: Quantitative real-time polymerase chain reaction was performed to analyze miR-30e-3p expression levels in AGS or BGC-823 cells 48 hours post transfection; B: CCK-8 assay was conducted to evaluate cell viability; C: Colony formation assay was conducted to evaluate the cell proliferation; D and E: Transwell assay was conducted using transwell chamber with or without matrigel to evaluate the cell migration (D) and invasion (E); F: Expression of E-cadherin, N-cadherin, and vimentin in AGS or BGC-823 was determined using western blotting. n = 3 for each group. ^aP < 0.05, ^bP < 0.01.