KLF16 promotes pancreatic adenocarcinoma cell proliferation and migration by positively regulating SMAD6

Figure 7 SMAD6 knockdown rescues the effects of Krüppel-like factor 16 overexpression on pancreatic adenocarcinoma. (A) Protein levels of Krüppel-like factor (KLF)16 and SMAD6 in control (Ctrl), KLF16, and KLF16+shSAMD6 cells by western blotting in MIA PaCa-2 cells. GAPDH used as the internal control; B: Cell viability of Ctrl, KLF16, and KLF16+shSAMD6 cells by CCK-8 assay in MIA PaCa-2 cells; C: Protein levels of KLF16 and SMAD6 in siCtrl, siKLF16, and siKLF16+SAMD6 cells by western blotting in AsPC-1 cells. GAPDH used as the internal control; D: Cell viability of siCtrl, siKLF16, and siKLF16+SAMD6 cells by the CCK-8 assay in AsPC-1 cells; E: Tumor size from three groups of nude mice that were injection with MIA PaCa-2 cells transfected with Ctrl, KLF16, and KLF16+shSAMD6, separately; F: Tumor growth curve in three groups; G: Tumor weight among the three groups. NS: Not significant. ^bP < 0.01; ^cP < 0.001.