Overexpression of ELL-associated factor 2 suppresses invasion, migration, and angiogenesis in colorectal cancer

Figure 8 Overexpression of ELL-associated factor 2 inhibited tumor-induced angiogenesis in RKO cells via the signal transducer and activator of transcription 3/transforming growth factor beta 1 pathway. A: Conditioned medium from RKO cells transfected with ELL-associated factor 2-overexpressed plasmids or treated with transforming growth factor beta 1 recombinant protein was collected to perform tube formation assay; B: Migration ability of human umbilical vein endothelial cells (HUVECs) analyzed by transwell migration assay; C: Migration ability of HUVECs analyzed by wound healing assay. And the results were measured with ImageJ software. The data are shown as the mean ± SD. ^aP < 0.05, ^bP < 0.001. RKO-C: The group of human umbilical vein endothelial cells cultured with conditioned medium from RKO cells cultured with 10% serum for 24 h; EAF2-OV: The group of human umbilical vein endothelial cells cultured with conditioned medium from RKO cells transfected with ELL-associated factor 2-overexpressed plasmids for 24 h; RKO + T: The group of human umbilical vein endothelial cells cultured with conditioned medium from RKO cells treated with 5 ng/mL transforming growth factor-β1 pathway recombinant protein for 24 h; EAF2-OV + T: The group of human umbilical vein endothelial cells cultured with conditioned medium from RKO cells which were transfected with EAF-OV plasmid for 24 h and then cultured with 5 ng/mL transforming growth factor beta 1 pathway recombinant protein for 24 h.