Overexpression of ELL-associated factor 2 suppresses invasion, migration, and angiogenesis in colorectal cancer

Figure 7 Overexpression of ELL-associated factor 2 in RKO cells inhibits tumor-induced angiogenesis. A: Conditioned medium from RKO cells transfected with ELL-associated factor 2-overexpressed plasmids or empty control plasmids was collected to perform tube formation assay; B: Migration ability of human umbilical vein endothelial cells (HUVECs) analyzed by transwell migration assay; C: Migration ability of HUVECs analyzed by wound healing assay. And the results were measured with ImageJ software. The data are shown as the mean ± SD. ^aP < 0.001. HUVECs: Human Umbilical Vein Endothelial Cells; RKO-C: The group of Human Umbilical Vein Endothelial Cells cultured with conditioned medium from RKO cells cultured with 10% serum for 24 h; EAF2-OV: The group of human umbilical vein endothelial cells cultured with conditioned medium from RKO cells transfected with ELL-associated factor 2-overexpressed plasmids for 24 h; EAF2-NC: The group of human umbilical vein endothelial cells cultured with conditioned medium from RKO cells transfected with empty control plasmids for 24 h.