Overexpression of ELL-associated factor 2 suppresses invasion, migration, and angiogenesis in colorectal cancer

Figure 4 Overexpression of ELL-associated factor 2 inhibits the invasion and migration of RKO cells. A: Protein levels of ELL-associated factor 2 (EAF2) in RKO cells transfected with EAF2 overexpression vector by western blot assay; B: Invasion and migration abilities evaluated by transwell assay; C: Migration ability analyzed by wound healing assay after 72 h; D: Migration and invasion abilities of RKO cells measured using ImageJ software. The data are shown as the mean ± SD. ^aP < 0.001; ^bP < 0.05. RKO-C: The group of RKO control cells; EAF2-OV: The group of RKO cells transfected with ELL-associated factor 2 overexpression vector; EAF2-NC: The group of RKO cells transfected empty vector; EAF2: ELL-associated factor 2; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.