Transcriptional factor III A promotes colorectal cancer progression by upregulating cystatin A

Figure 2 Knockdown of transcriptional factor III A gene inhibits colorectal cancer cell proliferation. A: The expression of transcriptional factor III A (GTF3A) in HCT116, SW480, DLD-1, SW620, and HT-29 cells was detected by Western blot; B: HCT116 and SW480 cells were stably transfected with short hairpin (sh) shscramble and shGTF3A#1 and #4-GV112 virus, respectively. Gtf3a mRNA (b1) and GTF3A protein (b2) expression were detected using real-time quantitative polymerase chain reaction and Western blot, respectively. The cell viability of HCT116 and SW480-shscramble as well as shGTF3A#1 and #4 cells was detected using Cell Counting Kit assay (b3); C: Colony formation of the transfected HCT116 and SW480 cells was detected using colony formation assay (c1), and the colony number was counted (c2). Gtf3a mRNA expression, cell colony number, and cell viability are expressed as the mean ± SEM of three independent experiments. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. GTF3A: Transcriptional factor III A; GAPDH: Glyceraldehydes-3-phosphate dehydrogenase.