Hydrogen-rich water exerts anti-tumor effects comparable to 5-fluorouracil in a colorectal cancer xenograft model

Figure 4 Hydrogen and 5-fluorouracil attenuate fibrosis in tumor tissue of colorectal cancer. A: Trichrome staining of tumor samples revealed that hydrogen (H₂) suppresses fibrosis in the murine model of colorectal cancer (collagen fiber accumulation appears in blue); B: Tumor fibrosis expressed as collagen content (%) in different groups. P < 0.001 compared to control, P < 0.001 compared to 5-fluorouracil, P <0.01 compared to H₂ groups; n = 6 per group. a: P < 0.001 compare to control; b: P < 0.001 compare to 5-FU; c: P < 0.001 compare to H2. H₂: Hydrogen; 5-FU: 5-fluorouracil.