Hydrogen-rich water exerts anti-tumor effects comparable to 5-fluorouracil in a colorectal cancer xenograft model

Figure 3 Hydrogen and 5-fluorouracil induce necrosis in tumor tissue of colorectal cancer. A: Hematoxylin-Eosin staining of tumor sections revealed that hydrogen (H₂) and 5-fluorouracil (5-FU) induce necrosis; B: Percent of tumor necrosis. P < 0.05, P < 0.01 and P < 0.001 compared to control, P < 0.001 compared to 5-FU, P < 0.01 compared to H₂ groups; n = 6 per group. a: P < 0.05 compare to control; b: P < 0.01 compare to control; c: P < 0.001 compare to control; d: P < 0.001 compare to 5-FU. H₂: Hydrogen; 5-FU: 5-fluorouracil; T: Tumor cells; N: Necrotic area.