Scoparone inhibits pancreatic cancer through PI3K/Akt signaling pathway

Figure 4 Scoparone causes cell cycle arrest and induces apoptosis. A-C: Scoparone causes G0/G1 phase arrest of Capan-2 and SW1990 cells by flow cytometry. The abundance of Capan-2 (B) and SW1990 (C) cells increased in the G0/G1 phase and decreased in the S phase compared with the control group; D-F: Scoparone induces the apoptosis of Capan-2 (E) and SW1990 (F) cells. The 100, 200, and 400 μmol/L scoparone groups had significantly higher apoptosis rates than the control group; G-J: Protein levels of MMP9, Bcl-2, Bax, and cleaved caspase-3 compared with those of the internal control GAPDH in Capan-2 (G and H) and SW1990 (I and J) cells. Decreased MMP9 and Bcl-2 expression and increased Bax and cleaved caspase-3 expression were observed. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001 vs the control group.