BRAFV600E mutant colorectal cancer cells mediate local immunosuppressive microenvironment through exosomal long noncoding RNAs

Figure 3 Effect of BRAF^V600E mutant colorectal cancer-cell-derived exosomes on the proliferation, migration, tube formation, and protein expression of human umbilical vein endothelial cells and human lymphatic endothelial cells. A: Detection of changes in proliferation of human umbilical vein endothelial cells (HUVECs) induced by exosomes derived from HT29 and 1627 cells at 24, 48, and 72 h by CCK8 assays. Changes in the proliferation of human lymphatic endothelial cells (HLECs) induced by exosomes derived from HT29 and 1627 cells were detected at 12, 24, and 36 h (the control group was treated with PBS); B: Detection of effects of HT29 and 1627 cell-derived exosomes on the migration of HUVECs and HLECs by scratch test (× 50); C: After 24 h of incubation, the effect of HT29 and 1627 cell-derived exosomes on tube formation ability of HUVECs and HLECs was observed (× 100); D: ELISA was used to detect expression of VEGF-A, TGF-β1, bFGF, and VEGF-C proteins in exosomes derived from HT29 and 1627 cells; E: ELISA was used to detect the effect of exosomes derived from HT29 and 1627 cells on expression of ZO-1 proteins in HUVECs; F: ELISA was used to detect the effect of exosomes derived from HT29 and 1627 cells on expression of ZO-1, occludin, and claudin-5 proteins in HLECs. ^aP < 0.05. HUVECs: Human umbilical vein endothelial cells; HLECs: Human lymphatic endothelial cells.