Genome-wide CRISPR-Cas9 screening identifies that hypoxia-inducible factor-1a-induced CBX8 transcription promotes pancreatic cancer progression via IRS1/AKT axis

Figure 4 Hypoxia-inducible factor-1α modulates CBX8 expression transcriptionally in pancreatic cancer cells. A: Human CBX8 promoter shown with HRE; B: 293T cells transfected with full HRE promoter and mutant HRE were cultured under normoxia or hypoxia for 48 h, and firefly luciferase activity determined relative to control Renilla luciferase; C: Quantitative reverse transcription-polymerase chain reaction for detecting hypoxia-inducible factor-1α (HIF-1α) knockdown efficiency under hypoxia; D: Expression level of CBX8 mRNA was evaluated and normalized to control group; E: Chromatin immunoprecipitation-qPCR analysis was used to determine the binding affinity of HIF-1α to CBX8 promoter regions; F and G: Expression levels of HIF-1α and CBX8 protein were evaluated and normalized to control group. ^bP < 0.01.