Genome-wide CRISPR-Cas9 screening identifies that hypoxia-inducible factor-1a-induced CBX8 transcription promotes pancreatic cancer progression via IRS1/AKT axis

Figure 1 Genome-wide pooled sgRNA library screening identifies genes affecting growth of pancreatic cancer cells in vivo. A: Schematic overview of experimental timeline and procedures of CRISPR/Cas9-based screening; B: Respective sgRNAs scored in negative selection CRISPR/Cas9 screening; C: Relative mRNA levels of top six sgRNAs detected by qPCR; D: KEGG pathway analysis of sgRNAs in negative selection CRISPR/Cas9 screening; E: GSEA of sgRNAs in the PI3K/AKT pathway.