Direct interaction between Rab5a and Rab4a enhanced epidermal growth factor-stimulated proliferation of gastric cancer cells

Figure 3 Overexpression of Rab5a and Rab4a promotes epidermal growth factor-induced proliferation of HGC-27 cells. Three groups of Control, Rab5a overexpression, and Rab5a + Rab4a overexpression were compared. Each group was analyzed with epidermal growth factor (EGF) or without (No-EGF) the presence of EGF treatment. A: A representative image of unstained cells (200 × magnification). The number in the upper right corner represents the cell concentration. Scale bar: 50 μm; B: Analysis of HGC-27 cell concentration was performed using ImageJ software based on optical densities. Cell concentrations are expressed as percentages. The bars represent mean values and the mean ± standard error calculated based on 10 representative images from each group (n = 10). The asterisks indicate ^aP < 0.05; C: Cell proliferation of each group was analyzed by cell counting kit-8 assay. The results showed that overexpression of Rab5a increased the proliferation of HGC-27 cells, and this effect was further enhanced by Rab4a overexpression. The asterisks indicate P < 0.05 (n = 3).