Interleukin-1 receptor antagonist enhances chemosensitivity to fluorouracil in treatment of Kras mutant colon cancer

Figure 6 Changes in colony formation in the SW620 and HCT116 cell lines treated with interleukin-1RA and/or fluorouracil. A: Colony formation assay showed that interleukin (IL)-1RA single drug treatment decreased the colony formation of SW620 cells, while this parameter significantly decreased in the SW620 cells treated with 6.25 mg/mL fluorouracil (5-FU) combined with 200 mg/mLIL-1RA compared with the untreated cells (5-FU vs Ctrl, P = 0.0027; IL-1RA vs Ctrl, ^aP = 0.0226; 5-FU and IL-1RA vs IL-1RA, ^bP = 0.0178; comparison of multiple groups: P = 0.0016); B: Colony formation assay showed that IL-1RA single drug treatment decreased the colony formation of HCT116 cells, while this parameter significantly decreased in the HCT116 cells treated with 6.25 mg/mL 5-FU combined with 200 mg/mLIL-1RA compared with the untreated cells (5-FU vs Ctrl, P = 0.0104; IL-1RA vs Ctrl, ^aP = 0.0451; 5-FU and IL-1RA vs IL-1RA, ^bP = 0.0063; comparison of multiple groups: P = 0.0013).