Interleukin-1 receptor antagonist enhances chemosensitivity to fluorouracil in treatment of Kras mutant colon cancer

Figure 3 Expression of interleukin-1α, interleukin-1β, and other interleukins in the siRNA-P65 SW620 cell line. A: Reverse transcription-polymerase chain reaction (RT-PCR) assay showed the nuclear factor kappa-B (NF-Κb) pathway was downregulated in the SW620 cell line after siRNA1 interference at 24 h (SW620 siRNA1 vs SW620, ^aP = 0.0003); B RT-PCR assay showed the NF-κB pathway was downregulated in the SW620 cell line after siRNA2 treatment at 24 h (SW620 siRNA2 vs SW620, ^aP = 0.0001); C: RT-PCR assay showed that the expression of IL-1α was significantly decreased in the SW620 siNF-κB cell line compared with the SW620 non-siNF-κB cell line (SW620 siNF-κB1 vs SW620, ^aP = 0.0002); D: RT-PCR assay showed that the expression of IL-1β was significantly decreased in the SW620 siNF-κB cell line compared with the SW620 non-siNF-κB cell line (SW620 siNF-κB1 vs SW620, ^aP = 0.0012); E: RT-PCR assay showed that the expression of IL-6 was significantly decreased in the SW620 siNF-κB cell line compared with the SW620 non-siNF-κB cell line (SW620 siNF-κB1 vs SW620, ^aP = 0.0012); F: RT-PCR assay showed that the expression of IL-8 was significantly decreased in the SW620 siNF-κB cell line compared with the SW620 non-siNF-κB cell line (SW620 siNF-κB1 vs SW620, ^aP = 0.0033); G: RT-PCR assay showed that the expression of IL-17 was significantly decreased in the SW620 siNF-κB cell line compared with the SW620 non-siNF-κB cell line (SW620 siNF-κB1 vs SW620, ^aP < 0.0001); H: RT-PCR assay showed that the expression of IL-21 was significantly decreased in the SW620 siNF-κB cell line compared with the SW620 non-siNF-κB cell line (SW620 siNF-κB1 vs SW620, ^aP = 0.0004); I: RT-PCR assay showed that the expression of TLR4 was significantly decreased in the SW620 siNF-κB cell line compared with the SW620 non-siNF-κB cell line (SW620 siNF-κB1 vs SW620, ^aP < 0.0001).