Interleukin-1 receptor antagonist enhances chemosensitivity to fluorouracil in treatment of Kras mutant colon cancer

Figure 1 Expression of phosphorylated nuclear factor kappa-B in Kras mutant colon carcinoma cell lines. A: Reverse transcription-polymerase chain reaction assays showed that the expression of the Kras gene in the COLO205, SW480, HT-29, LoVo, HCT116, DLD1, SW620, LS174T, and SW1116 cell lines was high. The expression level of Kras in the COLO205 cell line was significantly higher than that in NCM460 (^aP < 0.0001, ^bP = 0.0001 vs NCM460; comparison of multiple groups: P < 0.0001); B and C: Western blot assays showed that the expression levels of phosphorylated nuclear factor kappa-B (NF-κB) in the COLO205, SW620, and HCT116 cell lines were increased compared with that of NCM460 (COLO205 vs NCM460, ^aP = 0.0467; SW620 vs NCM460, ^bP = 0.0050; HCT116 vs NCM460, ^cP = 0.0177; comparison of multiple groups: P = 0.0081); D and E: Western blot assays showed that the expression of interleukin-1α was higher in the COLO205, SW620, and HCT116 cell lines than the NCM460 cell line (COLO205 vs NCM460, ^aP = 0.0427; SW620 vs NCM460, ^bP = 0.0100; HCT116 vs NCM460, ^cP = 0.0024; comparison of multiple groups: P = 0.0019).