LINC00511 promotes gastric cancer cell growth by acting as a ceRNA

Figure 4 PDK4 is a downstream target of miR-124-3p. A: The expression level of PDK4 in gastric cancer (GC) cells and normal gastric cells was determined by quantitative real-time PCR; B, C: Quantitative real-time PCR and WB assays were utilized to examine the expression level of PDK4 after miR-124-3p overexpression; D: The binding site of miR-124-3p and PDK4 was predicted with starBase v2.0; E: A luciferase reporter assay was used to verify that miR-124-3p could sponge PDK4; F: WB assay showed that LINC00511 silencing resulted in a reduction of PDK4 protein levels, which was partly rescued by miR-124-3p inhibition. ^aP < 0.05, ^bP < 0.01.